Author:
Hakenbeck R,Tarpay M,Tomasz A
Abstract
Penicillin-binding properties and characteristics of penicillin-binding proteins (PBPs) were investigated in several clinical isolates of Streptococcus pneumoniae differing in their susceptibilities to penicillin (minimal inhibitory concentration [MIC], 0.03 to 0.5 microgram/ml) and compared with the penicillin-susceptible strain R36A (MIC, 0.07 microgram/ml). Several changes accompanied the development of resistance: the relative affinity to penicillin of whole cells, isolated membranes, and two major PBPs after in vivo or in vitro labeling decreased (with increasing resistance). Furthermore, one additional PBP (2') appeared in four of five relatively resistant strains with an MIC of 0.25 microgram/ml and higher. PBP 3 maintained the same high affinity toward penicillin in all strains under all labeling conditions.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Reference30 articles.
1. Barry A. L. and L. D. Sabath. Special tests: bactericidal activity and activity of antimicrobics in combination p. 431-435. In E. H. Lennette E. H. Spaulding and J. P. Truant (ed.) Manual of clinical microbiology 2nd ed. American Society for Microbiology Washington D.C.
2. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels;Bonner W. M.;Eur. J. Biochem.,1974
3. Altered penicillin binding components in penicillin-resistant mutants of Bacillus subtilis;Buchanan C. E.;Proc. Natl. Acad. Sci. U.S.A.,1976
4. The binding of penicillin in relation to its cytotoxic action. I. Correlation between the penicillin sensitivity and combining activity of intact bacteria and cell free extracts;Eagle H.;J. Esp. Med.,1954
5. The binding of peniciLlin in relation to its cytotoxic action. II. The reactivity with penicillin of resistant variants of Streptococci, Pneumococci, and Staphylococci;Eagle H.;J. Exp. Med.,1954
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