Positioning of Region 4 of the Escherichia coli RNA Polymerase ς 70 Subunit by a Transcription Activator-Reference-Cited by-同舟云学术

Positioning of Region 4 of the Escherichia coli RNA Polymerase ς 70 Subunit by a Transcription Activator

Published:2000-05-15 Issue:10 Volume:182 Page:2982-2984
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Bown Jonathan A.¹,Kolb Annie²,Meares Claude F.³,Ishihama Akira⁴,Minchin Stephen D.¹,Busby Stephen J. W.¹

Affiliation:

1. School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom1;

2. Institut Pasteur, Unitéde Physico-chimie des Macromolécules Biologiques, 75724 Paris Cedex 15, France2;

3. Department of Chemistry, University of California, Davis, California 956163; and

4. Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411, Japan4

Abstract

ABSTRACT A DNA cleavage reagent, specifically tethered to residue 581 of the Escherichia coli RNA polymerase ς 70 subunit, has been used to investigate the location of ς 70 region 4 in different complexes at the galp 1 promoter and the effect of the cyclic AMP receptor protein. The positions of DNA cleavage by the reagent are not affected by the cyclic AMP receptor protein. We conclude that transcription activation at the galp 1 promoter by the cyclic AMP receptor protein does not involve major conformation changes in or repositioning of ς 70 region 4.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.182.10.2982-2984.2000

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