Assessment of flhDC mRNA Levels in Serratia liquefaciens Swarm Cells-Reference-Cited by-同舟云学术

Assessment of flhDC mRNA Levels in Serratia liquefaciens Swarm Cells

Published:2000-05-15 Issue:10 Volume:182 Page:2680-2686
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Tolker-Nielsen Tim¹,Christensen Allan Beck¹,Holmstrøm Kim²,Eberl Leo³,Rasmussen Thomas Bovbjerg¹,Sternberg Claus¹,Heydorn Arne¹,Molin Søren¹,Givskov Michael¹

Affiliation:

1. Department of Microbiology, The Technical University of Denmark, DK-2800 Lyngby,1 and

2. Biotechnological Institute, DK-2970 Hørsholm,2 Denmark, and

3. Lehrstuhl für Mikrobiologie, Technische Universität München, D-80290 Munich, Germany3

Abstract

ABSTRACT We reported previously that artificial overexpression of the flhDC operon in liquid-grown Serratia liquefaciens resulted in the formation of filamentous, multinucleated, and hyperflagellated cells that were indistinguishable from surface-induced swarm cells (L. Eberl, G. Christiansen, S. Molin, and M. Givskov, J. Bacteriol. 178:554–559, 1996). In the present report we show by means of reporter gene measurements, Northern analysis, and in situ reverse transcription-PCR that the amount of flhDC mRNA in surface-grown swarm cells does not exceed the maximum level found in nondifferentiated, vegetative cells. This suggests that surface-induced S. liquefaciens swarm cell differentiation, although dependent on flhDC gene expression, does not occur through elevated flhDC mRNA levels.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.182.10.2680-2686.2000

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