Western Blot Assay Using Recombinant p26 Antigen for Detection of Equine Infectious Anemia Virus-Specific Antibodies-Reference-Cited by-同舟云学术

Western Blot Assay Using Recombinant p26 Antigen for Detection of Equine Infectious Anemia Virus-Specific Antibodies

Published:2007-12 Issue:12 Volume:14 Page:1646-1648
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

Alvarez I.¹²,Gutierrez G.¹²,Ostlund E.¹²,Barrandeguy M.¹²,Trono K.¹²

Affiliation:

1. Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria, C.C. 1712, Castelar, Argentina

2. National Veterinary Services Laboratories, P.O. Box 844, Ames, Iowa 50010

Abstract

ABSTRACT We analyzed the performance of a single-band Western blot (WB) test using recombinant p26 (rp26) capsid protein of equine infectious anemia virus. According to the results obtained, the rp26 WB test is a reliable confirmatory diagnostic tool to be used as a complementary test after an enzyme-linked immunosorbent assay or agar gel immunodiffusion test yielding doubtful results.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CVI.00293-07

Reference13 articles.

1. Alvarez, I., G. Gutierrez, A. Vissani, S. Rodriguez, M. Barrandeguy, and K. Trono. 2007. Standardization and validation of an agar gel immunodiffusion test for the diagnosis of equine infectious anemia using a recombinant p26 antigen. Vet. Microbiol.121:344-351.

2. Development of an enzyme-linked immunosorbent assay for equine infectious anemia virus detection using recombinant Pr55gag

3. Bürki, F., W. Rossmanith, and E. Rossmanith. 1992. Equine lentivirus, comparative studies on four serological tests for the diagnosis of equine infectious anaemia. Vet. Microbiol.33:353-360.

4. Equine infectious anemia: 2001 update. 2001

5. Maturation of the cellular and humoral immune responses to persistent infection in horses by equine infectious anemia virus is a complex and lengthy process

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