Evaluation of the PACE 2 Neisseria gonorrhoeae assay by three public health laboratories

Author:

Hale Y M1,Melton M E1,Lewis J S1,Willis D E1

Affiliation:

1. Office of Laboratory Services, State of Florida, Health and Rehabilitative Services, Jacksonville 32202.

Abstract

The Gen-Probe PACE 2 DNA probe assay for Neisseria gonorrhoeae was compared with conventional culture techniques in three Florida public health laboratories with 436 patients (271 females and 165 males). The prevalence rates based on culture were 19.9, 55.8, and 33.5% for females, for males, and overall, respectively. Twenty-seven probe-positive specimens gave negative culture results. Twenty of these specimens were resolved as true positives after retesting with a probe competition assay. The resolved sensitivity, specificity, positive predictive value, and negative predictive value were 99.4, 99.6, 99.4, and 99.6%, respectively.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference7 articles.

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