The Tetraspanin CD81 Is a Host Factor for Chikungunya Virus Replication

Author:

Lasswitz Lisa12ORCID,Zapatero-Belinchón Francisco J.1234,Moeller Rebecca12,Hülskötter Kirsten5,Laurent Timothée367,Carlson Lars-Anders367,Goffinet Christine89,Simmons Graham1011,Baumgärtner Wolfgang5,Gerold Gisa1234ORCID

Affiliation:

1. Institute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hanover, Germany

2. Institute for Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hanover, Hanover, Germany

3. Wallenberg Centre for Molecular Medicine (WCMM), Umeå University, Umeå, Sweden

4. Department of Clinical Microbiology, Virology, Umeå University, Umeå, Sweden

5. Department of Pathology, University of Veterinary Medicine Hanover, Hanover, Germany

6. Department of Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden

7. Molecular Infection Medicine Sweden, Umeå University, Umeå, Sweden

8. Charité–Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Institute of Virology, Berlin, Germany

9. Berlin Institute of Health at Charité–Universitätsmedizin Berlin, Berlin, Germany

10. Vitalant Research Institute, University of California, San Francisco, California, USA

11. Department of Laboratory Medicine, University of California, San Francisco, California, USA

Abstract

In this study, we discover the tetraspanin CD81 as a host factor for the globally emerging chikungunya virus and related alphaviruses. We show that CD81 promotes replication of viral genomes in human and mouse cells, while virus entry into cells is independent of CD81.

Funder

Deutsche Forschungsgemeinschaft

Knut och Alice Wallenbergs Stiftelse

Infection Biology Ph.D. program of Hannover Biomedical Research School

HHS | National Institutes of Health

Impulse and Networking Fund of the Helmholtz Association

the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy - EXC 2155

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

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