The Plaque-Antiserum Method: an Assay of Virus Infectivity and an Experimental Model of Virus Infection

Author:

De Flora Silvio1

Affiliation:

1. Institute of Hygiene, University of Genoa, 16132 Genoa, Italy

Abstract

Areas of cytopathic effect can be circumscribed in cell monolayers by adding antiserum to the liquid nutrient medium after adsorption of virus. This procedure represents a simple and reliable tool for the titration of virus infectivity and provides an experimental model for studying some aspects of virus infection.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference12 articles.

1. Baron S. 1970. Host defenses during virus infection p. 77-110. In R. B. Heath and A. R. Waterson (ed.) Modern trends in medical virology. Butterworths London.

2. Micro-epidemiology of poliomyelitis and herpes-B infections;Black F. L.;J. Immunol.,1955

3. Cooper P. D. 1967. The plaque assay of animal viruses p. 243-311. In K. Maramorosch and H. Koprowski (ed.) Methods in virology vol. 3. Academic Press Inc. New York.

4. Study of plaques formed by vaccinia virus on SIRC cells;De Flora S.;Boll. Ist. Sieroter. Milan.,1969

5. A new technique for the formation of plaques yielded by polioviruses;De Flora S.;G. Ig. Med. Prev.,1969

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