Basic Studies for Continuous Production of L-Aspartic Acid by Immobilized Escherichia coli Cells-Reference-Cited by-同舟云学术

Basic Studies for Continuous Production of L-Aspartic Acid by Immobilized Escherichia coli Cells

Published:1974-05 Issue:5 Volume:27 Page:886-889
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Tosa Tetsuya¹,Sato Tadashi¹,Mori Takao¹,Chibata Ichiro¹

Affiliation:

1. Department of Biochemistry, Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd., Kashima-cho, Higashiyodogawa-ku, Osaka, Japan

Abstract

By using a column packed with immobilized Escherichia coli cells entrapped in a polyacrylamide gel lattice, conditions for continuous production of L-aspartic acid from ammonium fumarate were investigated. When a solution of 1 M ammonium fumarate (pH 8.5) containing 1 mM Mg 2+ was passed through the immobilized cell column at a flow rate of space velocity (SV) = 0.8 at 37 C, the highest rate of reaction was attained. From the column effluents, L-aspartic acid was obtained in good yield. The immobilized cell column was very stable.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.27.5.886-889.1974

Reference6 articles.

1. Immobilized aspartase-containing microbial cells: preparation and enzymatic properties;Chibata I.;Appl. Microbiol.,1974

2. A uniform medium for determination of amino acids with various microorganisms;Henderson L. M.;J. Biol. Chem.,1948

3. Production of aspartic acid from fumaric acid by microorganism;Kinoshita S.;Hakko Kyokaishi,1958

4. Studies on the fermentative preparation of L-aspartic acid from fumaric acid;Kisumi M.;Bull. Agr. Chem. Soc. Jap.,1960

5. Preparation of L-aspartic acid by bacterial aspartase;Kitahara K.;J. Agr. Chem. Soc. Jap.,1960

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