Mechanism through Which Retrocyclin Targets Flavivirus Multiplication-Reference-Cited by-同舟云学术

Mechanism through Which Retrocyclin Targets Flavivirus Multiplication

Published:2021-07-12 Issue:15 Volume:95 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Jia Xiaoying¹²,Guo Jiao¹²,Yuan Weirong³,Sun Lingling³,Liu Yang¹,Zhou Minmin¹²,Xiao Gengfu¹²,Lu Wuyuan³,Garzino-Demo Alfredo³⁴^ORCID,Wang Wei¹²^ORCID

Affiliation:

1. State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China

2. University of the Chinese Academy of Sciences, Beijing, China

3. Department of Microbiology and Immunology, the Institute of Human Virology, University of Maryland School of Medicine, Baltimore, Maryland, USA

4. Department of Molecular Medicine, University of Padua, Padua, Italy

Abstract

Retrocyclin is an artificially humanized circular θ-defensin peptide, containing 18 residues, previously reported to possess broad antimicrobial activity. In this study, we found that retrocyclin-101 inhibited flavivirus (ZIKV and JEV) infections. Retrocyclin-101 inhibited NS2B-NS3 serine protease activity, suggesting that the catalytic triad of the protease is the target. Moreover, retrocyclin-101 bound to the DE loop of the E protein of flavivirus, which prevented its entry.

Funder

The National Key Research and Development Program of China

Wuhan National Biosafety Laboratory,Chinese Academy of Sciences Advanced Customer Cultivation Project

The Open Research Fund Program of the state Key Laboratory of Virology of China

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.00560-21

Reference55 articles.