Existence of Hepatitis C Virus in Culex quinquefasciatus after Ingestion of Infected Blood: Experimental Approach to Evaluating Transmission by Mosquitoes

Author:

Chang Ting-Tsung1,Chang Tsuey-Yu2,Chen Cheng-Chen3,Young Kung-Chia4,Roan Jun-Neng5,Lee Yen-Chien5,Cheng Pin-Nan5,Wu Hua-Lin6

Affiliation:

1. Institute of Clinical Medicine,1

2. Department of Parasitology,2

3. Department of Parasitology, National Yang Ming University, Taipei,3 Taiwan, Republic of China

4. Department of Medical Technology,4

5. Department of Medicine,5 and

6. Department of Biochemistry,6 College of Medicine, National Cheng Kung University, Tainan, and

Abstract

ABSTRACT We used PCR to detect hepatitis C virus (HCV) RNA among supernatants of ground Culex quinquefasciatus mosquitoes that (i) had been fed HCV-positive blood, (ii) had been intrathoracically inoculated with HCV-positive blood, or (iii) were from homes of hepatitis C patients. HCV RNA was detectable under all three conditions, but it did not replicate in mosquitoes and was not detectably transmitted during feeding.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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