Sensitivity and Specificity of a Rapid rRNA Gene Probe Assay for Simultaneous Identification of Staphylococcus aureus and Detection of mecA

Author:

Kaplan Shannon1,Marlowe Elizabeth M.1,Hogan James J.1,Doymaz Mehmet2,Bruckner David A.2,Simor Andrew E.3

Affiliation:

1. Gen-Probe Incorporated, San Diego, California

2. UCLA Medical Center, Department of Pathology and Laboratory Medicine, Los Angeles, California

3. Sunnybrook and Women's College Health Sciences Centre, Department of Microbiology, Toronto, Canada

Abstract

ABSTRACT rRNA gene sequences were used for identification and target adequacy controls in a DNA probe assay to identify isolates as Staphylococcus and, more specifically, as S. aureus within 1 hour. mecA status was simultaneously determined using a specific DNA probe. The target adequacy control guarded against false-negative mecA results.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Business of Microbiology;Practical Handbook of Microbiology, Third Edition;2015-05-28

2. Update of hybridization-based methods for the rapid diagnosis of sepsis;Reviews in Medical Microbiology;2010-10

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