Sensitivity and Specificity of a Rapid rRNA Gene Probe Assay for Simultaneous Identification of Staphylococcus aureus and Detection of mecA-Reference-Cited by-同舟云学术

Sensitivity and Specificity of a Rapid rRNA Gene Probe Assay for Simultaneous Identification of Staphylococcus aureus and Detection of mecA

Published:2005-07 Issue:7 Volume:43 Page:3438-3442
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Kaplan Shannon¹,Marlowe Elizabeth M.¹,Hogan James J.¹,Doymaz Mehmet²,Bruckner David A.²,Simor Andrew E.³

Affiliation:

1. Gen-Probe Incorporated, San Diego, California

2. UCLA Medical Center, Department of Pathology and Laboratory Medicine, Los Angeles, California

3. Sunnybrook and Women's College Health Sciences Centre, Department of Microbiology, Toronto, Canada

Abstract

ABSTRACT rRNA gene sequences were used for identification and target adequacy controls in a DNA probe assay to identify isolates as Staphylococcus and, more specifically, as S. aureus within 1 hour. mecA status was simultaneously determined using a specific DNA probe. The target adequacy control guarded against false-negative mecA results.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.43.7.3438-3442.2005

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