Identification of Clinical Aeromonas Species byrpoBandgyrBSequencing and Development of a Multiplex PCR Method for Detection of Aeromonas hydrophila, A. caviae, A. veronii, and A. media

Abstract

Conventional identification ofAeromonasspecies based on biochemical methods is challenged by the heterogeneous nature of the species. Here, we present a new multiplex PCR method directed toward thegyrBandrpoBgenes that identifies fourAeromonasspecies,A. hydrophila,A. media,A. veronii, andA. caviae, and we describe the application of this method on a Danish strain collection.