Evaluation of Cell-Based and Surrogate SARS-CoV-2 Neutralization Assays

Author:

Sholukh Anton M.1ORCID,Fiore-Gartland Andrew1ORCID,Ford Emily S.12,Miner Maurine D.1,Hou Yixuan J.3,Tse Longping V.3,Kaiser Hannah4,Zhu Haiying5,Lu Joyce5,Madarampalli Bhanupriya5,Park Arnold4ORCID,Lempp Florian A.4,St. Germain Russell1,Bossard Emily L.1,Kee Jia Jin1,Diem Kurt5,Stuart Andrew B.1,Rupert Peter B.6,Brock Chance6,Buerger Matthew6,Doll Margaret K.7,Randhawa April Kaur1,Stamatatos Leonidas1,Strong Roland K.16,McLaughlin Colleen7,Huang Meei-Li5,Jerome Keith R.15,Baric Ralph S.38ORCID,Montefiori David910,Corey Lawrence125

Affiliation:

1. Vaccine and Infectious Diseases Division, Fred Hutch Cancer Research Center, Seattle, Washington, USA

2. Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington, USA

3. Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

4. Vir Biotechnology, San Francisco, California, USA

5. Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, USA

6. Basic Sciences Division, Fred Hutch Cancer Research Center, Seattle, Washington, USA

7. Department of Population Health Sciences, Albany College of Pharmacy and Health Sciences, Albany, New York, USA

8. Department of Microbiology and Immunology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

9. Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina, USA

10. Department of Surgery, Duke University, Durham, North Carolina, USA

Abstract

Determinants of protective immunity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection require the development of well-standardized, reproducible antibody assays. This need has led to the emergence of a variety of neutralization assays.

Funder

Fred Hutchinson Cancer Research Center

HHS | NIH | National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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