Affiliation:
1. Saitama Institute of Public Health, Omiya, Saitama, Japan
Abstract
A group of enzymes were prepared from the culture fluids of streptococci belonging to groups A, B, C, G, and L, and from a strain of
Streptococcus sanguis
. These streptococcal enzymes (designated St-sialidases) released a substance shown to belong to the sialic acid group from the specific substrate BSM-St, a sialomucoid prepared from bovine submaxillary gland. They were inactive on
N
-acetylneuramin lactose prepared from bovine colustrum and on a sialomucoid prepared from bovine submaxillary mucin, whereas these substances are susceptible to sialidases produced by group K streptococci and by
Vibrio cholerae
. Some of the St-sialidases were markedly activated by divalent cations, but others showed little response. The heat stability of the enzymes produced by the different strains varied. The optimal
p
H was between 5.5 and 6.5 with acetate buffer and was about 7 with phosphate buffer.
K
m
values were determined for the St-sialidases with BSM-St as substrate.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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