Affiliation:
1. Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
2. Service de Microbiologie, CHU Côte de Nacre, 14033 Caen Cedex, France
3. Nakornping Hospital
4. Departments of Microbiology
5. Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand
Abstract
ABSTRACT
The antimicrobial susceptibilities of 30
Rhodococcus equi
isolates obtained from 30 patients between 1993 and 2001 in northern Thailand were investigated. The MICs showed a tendency toward resistance to various antibiotics but sensitivity to imipenem, minocycline, vancomycin, and teicoplanin (MICs, ≤0.5 μg/ml) and relative sensitivity to meropenem, clarithromycin, and ciprofloxacin (MICs, ≤2 μg/ml). Of the 30 isolates, 26 were susceptible (MICs, ≤1 μg/ml), 1 showed low-level resistance (MIC, 8 μg/ml), and 3 showed high-level resistance (MICs, ≥64 μg/ml) to rifampin. PCR amplification and DNA sequencing of the
rpoB
gene and molecular typing by pulsed-field gel electrophoresis (PFGE) were performed for eight
R. equi
isolates from eight AIDS patients with pneumonia or lung abscess caused by
R. equi
between 1998 and 2001, including one low- and three high-level rifampin-resistant isolates. As a result, two high-level rifampin-resistant strains with PFGE pattern A had a Ser531Trp (
Escherichia coli
numbering) mutation, and one high-level rifampin-resistant strain with PFGE pattern B had a His526Tyr mutation, whereas one low-level rifampin-resistant strain with PFGE pattern C had a Ser509Pro mutation. Four rifampin-susceptible strains with PFGE patterns D and E showed an absence of mutation in the
rpoB
region. Our results indicate the presence of several types of rifampin-resistant
R. equi
strains among AIDS patients in northern Thailand.
Publisher
American Society for Microbiology
Cited by
50 articles.
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