Subgenotype Analysis of Cryptosporidium Isolates from Humans, Cattle, and Zoo Ruminants in Portugal

Author:

Alves Margarida1,Xiao Lihua2,Sulaiman Irshad2,Lal Altaf A.2,Matos Olga1,Antunes Francisco13

Affiliation:

1. Unidade de Protozoários Oportunistas/VIH e Outras Protozooses, Unidade de Parasitologia e Microbiologia Médicas (UPMM), Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, 1349-008 Lisbon

2. Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341

3. Clínica Universitária de Doenças Infecciosas, Faculdade de Medicina (Hospital de Santa Maria), Universidade de Lisboa, 1600 Lisbon, Portugal

Abstract

ABSTRACT Cryptosporidium parvum and Cryptosporidium hominis isolates from human immunodeficiency virus-infected patients, cattle, and wild ruminants were characterized by PCR and DNA sequencing analysis of the 60-kDa glycoprotein gene. Seven alleles were identified, three corresponding to C. hominis and four corresponding to C. parvum . One new allele was found (IId), and one (IIb) had only been found in Portugal. Isolates from cattle and wild ruminants clustered in two alleles. In contrast, human isolates clustered in seven alleles, showing extensive allelic diversity.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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