On the Consequences of Poorly Defined Breakpoints for Rifampin Susceptibility Testing of Mycobacterium tuberculosis Complex

Author:

Köser Claudio U.1ORCID,Georghiou Sophia B.2,Schön Thomas345,Salfinger Max6

Affiliation:

1. Department of Genetics, University of Cambridge, Cambridge, United Kingdom

2. FIND, Geneva, Switzerland

3. Department of Infectious Diseases, Kalmar County Hospital, Linköping University, Kalmar, Sweden

4. Unit of Infection and Inflammation, Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden

5. Department of Infectious Diseases, Linköping University Hospital, Linköping, Sweden

6. University of South Florida College of Public Health and Morsani College of Medicine, Tampa, Florida, USA

Abstract

In a recent report of a systematic review of critical concentrations (CCs), the World Health Organization (WHO) lowered the rifampin (RIF) CC for antimicrobial susceptibility testing (AST) of the Mycobacterium tuberculosis complex using Middlebrook 7H10 medium and the Bactec mycobacterial growth indicator tube (MGIT) 960 system from 1 to 0.5 μg/ml. The previous RIF CC for 7H10 had been in use for over half a century.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference45 articles.

1. Rifampin in the Treatment of Drug-ResistantMycobacterium tuberculosisInfections

2. The Diagnosis and Therapy of Tuberculosis During the Past 100 Years

3. World Health Organization. 2020. Global tuberculosis report 2020. World Health Organization Geneva Switzerland. https://apps.who.int/iris/bitstream/handle/10665/336069/9789240013131-eng.pdf.

4. Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis

5. Targeted next-generation sequencing: a Swiss army knife for mycobacterial diagnostics?;Mohamed S;Eur Respir J

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