Affiliation:
1. Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina 27858-4354
Abstract
ABSTRACT
Brucella abortus
2308 derivatives with mini-Tn
5
insertions in
purE
,
purL
, and
purD
display significant attenuation in the BALB/c mouse model, while isogenic mutants with mini-Tn
5
insertions in
pheA
,
trpB
, and
dagA
display little or no attenuation in cultured murine macrophages or mice. These experimental findings confirm the importance of the purine biosynthesis pathways for the survival and replication of the brucellae in host macrophages. In contrast to previous reports, however, these results indicate that exogenous tryptophan and phenylalanine are available for use by the brucellae in the phagosomal compartment.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference36 articles.
1. Bakkestuen, A. K., L.-L. Gundersen, G. Langli, F. Liu, and J. M. J. Nolsøe. 2000. 9-Benzylpurines with inhibitory activity against Mycobacterium tuberculosis. Bioorg. Med. Chem. Lett.10:1207-1210.
2. Baldwin, C. L., and R. M. Roop II. 1999. Brucella infections and immunity, p. 255-279. In L. J. Paradise, H. Friedman, and M. Bendinelli (ed.), Opportunistic intracellular bacteria and immunity. Plenum Press, New York, N.Y.
3. Leucine auxotrophy restricts growth of Mycobacterium bovis BCG in macrophages
4. Bacterial persistence and immunity in goats vaccinated with a purE deletion mutant or the parental 16M strain of Brucella melitensis
5. Deletion of purE attenuates Brucella melitensis infection in mice
Cited by
57 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献