Affiliation:
1. Alimentary Pharmabiotic Centre
2. Departments of Microbiology
3. Food and Nutritional Sciences, Bioscience Institute, National University of Ireland, Cork, Western Road, Cork, Ireland
Abstract
ABSTRACT
Forty-two bifidobacterial strains were screened for α-amylase and/or pullulanase activity by investigating their capacities to utilize starch, amylopectin, or pullulan. Of the 42 bifidobacterial strains tested, 19 were capable of degrading potato starch. Of these 19 strains, 11 were able to degrade starch and amylopectin, as well as pullulan. These 11 strains, which were shown to produce extracellular starch-degrading activities, included 5 strains of
Bifidobacterium breve
, 1
B. dentium
strain, 1
B. infantis
strain, 3 strains of
B. pseudolongum
, and 1 strain of
B. thermophilum
. Quantitative and qualitative enzyme activities were determined by measuring the concentrations of released reducing sugars and by high-performance thin-layer chromatography, respectively. These analyses confirmed both the inducible nature and the extracellular nature of the starch- and pullulan-degrading enzyme activities and showed that the five
B. breve
strains produced an activity that is consistent with type II pullulanase (amylopullulanase) activity, while the remaining six strains produced an activity with properties that resemble those of type III pullulan hydrolase.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
141 articles.
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