Engineering and Characterization of Avian Coronavirus Mutants Expressing Fluorescent Reporter Proteins from the Replicase Gene-Reference-Cited by-同舟云学术

Engineering and Characterization of Avian Coronavirus Mutants Expressing Fluorescent Reporter Proteins from the Replicase Gene

Published:2022-07-27 Issue:14 Volume:96 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Xing Na¹,Wang Zhisheng²,Wang Jichun²,Nascimento Mariana¹,Jongkaewwattana Anan³,Trimpert Jakob¹,Osterrieder Nikolaus¹⁴,Kunec Dusan¹^ORCID

Affiliation:

1. Institut für Virologie, Freie Universität Berlin, Berlin, Germany

2. National Veterinary Product Engineering Research Center, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, China

3. National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency (NSTDA), Pathumthani, Thailand

4. Department of Infectious Disease and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong

Abstract

Reverse genetics systems based on bacterial artificial chromosomes (BACs) are the most valuable systems in coronavirus research. Here, we describe the establishment of a reverse genetics system for the avian coronavirus strain Beaudette, the most intensively studied strain.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.00653-22

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