Affiliation:
1. Department of Immunology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105
2. Program in Theoretical Biology, Institute for Advanced Study, Princeton, New Jersey 08540
Abstract
ABSTRACT
The primary influenza A virus-specific CD8
+
-T-cell responses measured by tetramer staining of spleen, lymph node, and bronchoalveolar lavage (BAL) lymphocyte populations were similar in magnitude for conventional
I-A
b
+/+
and CD4
+
-T-cell-deficient
I-A
b
−/−
mice. Comparable levels of virus-specific cytotoxic-T-lymphocyte activity were detected in the inflammatory exudate recovered by BAL following challenge. However, both the size of the memory T-cell pool and the magnitude of the recall response in the lymphoid tissues (but not the BAL specimens) were significantly diminished in mice lacking the CD4
+
subset. Also, the rate of virus elimination from the infected respiratory tract slowed at low virus loads following challenge of naïve and previously immunized
I-A
b
−/−
mice. Thus, though the capacity to mediate the CD8
+
-T-cell effector function is broadly preserved in the absence of concurrent CD4
+
-T-cell help, both the maintenance and recall of memory are compromised and the clearance of residual virus is delayed. These findings are consistent with mathematical models that predict virus-host dynamics in this, and other, models of infection.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
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+
T-Cell Response Is Much Less Apparent following Secondary Challenge
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