Multilocus Variable-Number Tandem-Repeat Analysis Scheme for Chlamydia felis Genotyping: Comparison with Multilocus Sequence Typing-Reference-Cited by-同舟云学术

Multilocus Variable-Number Tandem-Repeat Analysis Scheme for Chlamydia felis Genotyping: Comparison with Multilocus Sequence Typing

Published:2012-06 Issue:6 Volume:50 Page:1860-1866
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Laroucau Karine¹,Di Francesco Antonietta²,Vorimore Fabien¹,Thierry Simon¹,Pingret Jean Luc³,Bertin Claire¹,Willems Hermann⁴,Bölske Goran⁵,Harley Ross⁶

Affiliation:

1. Bacterial Zoonoses Unit, French Agency for Food, Environmental & Occupational Health Safety (ANSES), Maisons-Alfort, France

2. Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy

3. Scanelis, Colomiers, France

4. Clinic for Swine, Giessen, Germany

5. Department of Bacteriology, National Veterinary Institute (SVA), Uppsala, Sweden

6. School of Clinical Veterinary Sciences, University of Bristol, Bristol, United Kingdom

Abstract

ABSTRACT Chlamydia felis is an important ocular pathogen in cats worldwide. A multilocus variable-number tandem-repeat analysis (MLVA) system for the detection of tandem repeats across the whole genome of C. felis strain Fe/C-56 was developed. Nine selected genetic loci were tested by MLVA in 17 C. felis isolates, including the C. felis Baker vaccine strain, and 122 clinical samples from different geographic origins. Analysis of the results identified 25 distinct C. felis MLVA patterns. In parallel, a recently described multilocus sequence typing scheme for the typing of Chlamydia was applied to 13 clinical samples with 12 different C. felis MLVA patterns. Rare sequence differences were observed. Thus, the newly developed MLVA system provides a highly sensitive high-resolution test for the differentiation of C. felis isolates from different origins that is suitable for molecular epidemiological studies.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00417-12

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