Tyrosine Phosphorylation of CagA from Chinese
Helicobacter pylori
Isolates in AGS Gastric Epithelial Cells
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Published:2005-02
Issue:2
Volume:43
Page:786-790
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ISSN:0095-1137
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Container-title:Journal of Clinical Microbiology
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language:en
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Short-container-title:J Clin Microbiol
Author:
Zhang Youli1, Argent Richard H.2, Letley Darren P.2, Thomas Rachael J.2, Atherton John C.2
Affiliation:
1. Division of Gastroenterology, The Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China 2. Institute of Infection, Immunity, and Inflammation and Wolfson Digestive Diseases Centre, University of Nottingham, Queen's Medical Centre, Nottingham, United Kingdom
Abstract
ABSTRACT
Helicobacter pylori
strains possessing the
cag
pathogenicity island (PaI) are associated with the development of gastroduodenal diseases, including gastric cancer.
cag
PaI products induce the secretion of interleukin-8 (IL-8) from epithelial cells and facilitate the translocation of CagA into the cell cytosol. In East Asia, where the incidence of gastric cancer is high, most strains possess the
cag
PaI. To date, however, no
cag
PaI phenotypic data have been provided for strains isolated in mainland China. Here we used 31 Chinese strains to determine the genotypic and phenotypic status of the
cag
PaI. All strains possessed
cagA
and
cagE
, and we observed a variation in the length of
cagA
variable regions. Nucleotide sequencing of the
cagA
variable region revealed that CagA was of two types, a short “Western” form with two tyrosine phosphorylation sites and a longer “East Asian” form with three tyrosine phosphorylation sites. Coculture of strains with AGS epithelial cells showed that strains could induce IL-8 secretion from the cells and that CagA with three phosphorylation sites became more phosphorylated than that with two and could induce significantly (
P
< 0.001) more cells to elongate. We hypothesize that the preponderance of the more active East Asian form of
cagA
may underlie the high rate of gastric cancer in China.
Publisher
American Society for Microbiology
Subject
Microbiology (medical)
Reference26 articles.
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