Identification of Variable-Number Tandem-Repeat Loci in Leptospira interrogans Sensu Stricto-Reference-Cited by-同舟云学术

Identification of Variable-Number Tandem-Repeat Loci in Leptospira interrogans Sensu Stricto

Published:2005-02 Issue:2 Volume:43 Page:539-545
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Majed Z.¹,Bellenger E.¹,Postic D.¹,Pourcel C.²,Baranton G.¹,Picardeau M.¹

Affiliation:

1. Laboratoire des Spirochètes, Institut Pasteur, Paris

2. Laboratoire GPMS, Institut de Génétique et Microbiologie, Université Paris-Sud, Orsay, France

Abstract

ABSTRACT Leptospira interrogans sensu stricto is responsible for the most frequent and severe cases of human leptospirosis. The epidemiology and clinical features of leptospirosis are usually associated with the serovars and serogroups of Leptospira . Because of the difficulties associated with serological identification of Leptospira strains, we evaluated a novel PCR-based method for typing L. interrogans serovars. Based upon the genome sequence of L. interrogans serovar Lai type strain 5660, 44 loci were analyzed by PCR for their variability in size due to the presence of variable-number tandem repeats (VNTR). Seven VNTR loci were found to be powerful markers for serovar identification, epidemiology, and phylogenetic studies of L. interrogans . This rapid and easy method should greatly contribute to a better knowledge of the epidemiology of Leptospira .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.43.2.539-545.2005

Reference26 articles.

1. Benson, G. 1999. Tandem repeats Finder: a program to analyze DNA sequences. Nucleic Acids Res.27:573-580.

2. Brenner, D. J., A. F. Kaufmann, K. R. Sulzer, A. G. Steigerwalt, F. C. Rogers, and R. S. Weyant. 1999. Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies. Int. J. Syst. Bacteriol.49:839-858.

3. Denoeud, F., and G. Vergnaud. 2004. Identification of polymorphic tandem repeats by direct comparison of genome sequence from different bacterial strains: a web-based resource. BMC Bioinformatics12:4.

4. Ellinghausen, H. C., and W. G. McCullough. 1965. Nutrition of Leptospira pomona and growth of 13 other serotypes: fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80. Am. J. Vet. Res.26:45-51.

5. Faine S. B. Adler C. Bolin and P. Perolat. 1999. Leptospira and leptospirosis. MedScience Melbourne Australia.

Cited by 101 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Residual disinfectant effectively suppresses Legionella species in drinking water distribution systems supplied by surface water in Minnesota, USA;Science of The Total Environment;2024-08

2. The serological and genetic diversity of the Leptospira interrogans Icterohaemorrhagiae serogroup circulating in the UK;Journal of Veterinary Research;2023-12-01

3. A Canine Leptospirosis Clinical Case Due to Leptospira interrogans (Serogroup Icterohaemorrhagiae) in a Dog Kennel in Castelvetrano (Western Sicily, South Italy);Veterinary Sciences;2023-08-06

4. Genetic structure and diversity ofrfblocus of pathogenic species of genusLeptospira;2023-03-24

5. Effects of Temperature, Treatment, and Residual Disinfection on the Concentrations of Legionella Species And Total Bacteria in Public Water Systems Supplied by Surface Water;2023