Affiliation:
1. Department of Biological Sciences, Purdue University, Lafayette, Indiana 47907
Abstract
A quantitative assay employing
125
I-labeled antibody has been developed for
Bacillus cereus
T spore coat protein. Populations of antibody molecules with various affinities for inner or outer coat can be prepared by selective adsorption to and elution from different coat preparations. Adsorption to and elution from intact spores results in an antibody preparation at least 15 times more reactive to outer coat. This antibody is useful for measuring the time and extent of spore coat maturation, i.e., outer spore coat formation. Rifampin inhibits the increase in content of this coat antigen.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference27 articles.
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