Use of a New Dual-Antigen Enzyme-Linked Immunosorbent Assay To Detect and Characterize the Human Antibody Response to the Human Immunodeficiency Virus Type 2 Envelope gp125 and gp36 Glycoproteins-Reference-Cited by-同舟云学术

Use of a New Dual-Antigen Enzyme-Linked Immunosorbent Assay To Detect and Characterize the Human Antibody Response to the Human Immunodeficiency Virus Type 2 Envelope gp125 and gp36 Glycoproteins

Published:2006-02 Issue:2 Volume:44 Page:607-611
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Marcelino José Maria¹,Barroso Helena²³,Gonçalves Fátima⁴,Silva Sofia Marques¹,Novo Carlos¹,Gomes Perpétua²⁴,Camacho Ricardo⁴,Taveira Nuno²³

Affiliation:

1. UTPAM, Departamento de Biotecnologia, Instituto Nacional de Engenharia Tecnologia e Inovação, Lisboa, Portugal

2. Instituto Superior de Ciências da Saúde Egas Moniz, Monte de Caparica, Portugal

3. Unidade de Retrovírus e Infecções Associadas, Centro de Patogénese Molecular, Faculdade de Farmácia de Lisboa, Lisboa, Portugal

4. Laboratório de Virologia, Serviço de Imuno-Hemoterapia, Hospital Egas Moniz, Lisboa, Portugal

Abstract

ABSTRACT A dual-antigen enzyme-linked immunosorbent assay specific for human immunodeficiency virus type 2 (HIV-2) envelope proteins, ELISA-HIV2, was developed with two new recombinant polypeptides, rpC2-C3 and rgp36, derived from the HIV-2 envelope. The diagnostic performance was determined with HIV-2, HIV-1, and HIV-1/2 samples. Both polypeptides showed 100% specificity. Clinical sensitivity was 100% for rgp36 and 93.4% for rpC2-C3. ELISA-HIV2 may be used for the specific diagnosis and confirmation of HIV-2 infection.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.44.2.607-611.2006

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