Author:
Al-Tebrineh Jamal,Merrick Chester,Ryan David,Humpage Andrew,Bowling Lee,Neilan Brett A.
Abstract
ABSTRACTA cyanobacterial bloom impacted over 1,100 km of the Murray River, Australia, and its tributaries in 2009. Physicochemical conditions in the river were optimal to support a bloom at the time. The data suggest that at least three blooms occurred concurrently in different sections of the river, with each having a different community composition and associated cyanotoxin profile. Microscopic and genetic analyses suggested the presence of potentially toxicAnabaena circinalis,Microcystis flos-aquae, andCylindrospermopsis raciborskiiat many locations. Low concentrations of saxitoxins and cylindrospermopsin were detected inAnabaenaandCylindrospermopsispopulations. A multiplex quantitative PCR was used, employing novel oligonucleotide primers and fluorescent TaqMan probes, to examine bloom toxigenicity. This single reaction method identified the presence of the major cyanotoxin-producing species present in these environmental samples and also quantified the various toxin biosynthesis genes. A large number of cells present throughout the bloom were not potential toxin producers or were present in numbers below the limit of detection of the assay and therefore not an immediate health risk. Potential toxin-producing cells, possessing the cylindrospermopsin biosynthesis gene (cyrA), predominated early in the bloom, while those possessing the saxitoxin biosynthesis gene (sxtA) were more common toward its decline. In this study, the concentrations of cyanotoxins measured via enzyme-linked immunosorbent assay (ELISA) correlated positively with the respective toxin gene copy numbers, indicating that the molecular method may be used as a proxy for bloom risk assessment.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference44 articles.
1. A new multiplex qPCR targeting hepato- and neuro-toxigenic cyanobacteria of global significance. Harmful Algae;Al-Tebrineh
2. Detection of saxitoxin-producing cyanobacteria and Anabaena circinalis in environmental water blooms by quantitative PCR;Al-Tebrineh;Appl. Environ. Microbiol.,2010
3. PERMANOVA+ for PRIMER: guide to software and statistical methods;Anderson,2008
4. Identification of common noxious cyanobacteria. Part I – Nostocales. Urban Water Research Association of Australia research report no. 29;Baker,1991
5. Identification of common noxious cyanobacteria. Part II – Chroococcales, Oscillatoriales. Urban Water Research Association of Australia research report no. 46;Baker,1992
Cited by
65 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献