Genetic and Biochemical Characterization of a Novel Metallo-β-Lactamase, TMB-1, from an Achromobacter xylosoxidans Strain Isolated in Tripoli, Libya

Abstract

ABSTRACTAnAchromobacter xylosoxidansstrain from the Tripoli central hospital produced a unique metallo-β-lactamase, designated TMB-1, which is related to DIM-1 (62%) and GIM-1 (51%).blaTMB-1was embedded in a class 1 integron and located on the chromosome. The TMB-1 β-lactamase has lowerkcatvalues than both DIM-1 and GIM-1 with cephalosporins and carbapenems. TheKmvalues were more similar to those of GIM-1 than those of DIM-1, with the overallkcat/Kmvalues being lower than those for GIM-1 and DIM-1.