Affiliation:
1. Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032
Abstract
The process of osmotic shock, which has been used to release degradative enzymes from
Escherichia coli
, can be applied successfully to other members of the
Enterobacteriaceae
. Cyclic phosphodiesterase (3′-nucleotidase), 5′-nucleotidase (diphosphate sugar hydrolase), acid hexose phosphatase, and acid phenyl phosphatase are released from
Shigella, Enterobacter, Citrobacter
, and
Serratia
strains. Some strains of
Salmonella
also release these enzymes. Members of
Proteus
and
Providencia
groups fail to release enzymes when subjected to osmotic shock and do not show a lag in regrowth, although they do release their acid-soluble nucleotide pools. In contrast to
E. coli
, release of enzymes from other members of the
Enterobacteriaceae
studied is affected by growth conditions and strain of organism. None of the organisms was as stable to osmotic shock in exponential phase of growth as was
E. coli
. Exponential-phase cells of
Shigella, Enterobacter
, and
Citrobacter
could be shocked only with 0.5 m
m
MgCl
2
to prevent irreparable damage to the cells. These observations suggest that this group of degradative enzymes is probably loosely bound to the cytoplasmic membrane through the mediation of divalent cations.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
134 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献