Affiliation:
1. Department of Biophysics, Michigan State University, East Lansing, Michigan 48823
Abstract
The distribution of platinum ions within
Escherichia coli
after the induction of filaments with
cis
-Pt(NH
3
)
2
Cl
4
, and after growth inhibition by PtCl
6
2−
, has been determined with radioactive metal compounds (
191
Pt, with a half-life of approximately 3 days) by the simple chemical procedure of Roberts et al. In the filamentous cells, the platinum metal is associated with metabolic intermediates, nucleic acids, and cytoplasmic proteins; whereas, in inhibited cells, the platinum is combined only with the cytoplasmic protein. Similar experiments with gram-positive cells of
Bacillus cereus
and
Staphyloccus aureus
, which show no filamentous growth in the presence of
cis
-Pt(NH
3
)
2
Cl
4
, reveal that the metal complex does penetrate the cell wall and subsequently becomes bound predominantly by metabolic intermediates.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference6 articles.
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2. ROBERTS R. B. P. H. ABELSON D. B. COwIE E. T. BOLTON AND R. J. BRITIEN. 1955. Studies of biosynthesis in Escherichia coli. Carnegie Inst. Wash. PubI. 607.
3. Platinuminduced filamentous growth in Escherichia coli;ROSENBERG B., E.;J. Bacteriol.,1967
4. Inhibition of cell division in Escherichia coli by electrolysis products from a platinum electrode;ROSENBERG B., L.;Nature,1965
5. The inhibition of growth or cell division in Escherichia coli by different ionic species of platinum (IV) complexes;ROSENBERG B., L.;J. Biol. Chem.,1967
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