Inhibition of protein synthesis transiently stimulates initiation of minichromosome replication in Escherichia coli

Author:

Weinberger M1,Helmstetter C E1

Affiliation:

1. Department of Experimental Biology, Roswell Park Memorial Institute, Buffalo, New York 14263.

Abstract

Replication of oriC-dependent minichromosomes was found to be transiently stimulated when protein synthesis was inhibited by the addition of chloramphenicol. Initiation of replication was also induced by amino acid starvation of relA mutant strains and a nutritional upshift. The results are explained on the basis that these treatments rendered RNA polymerase more available for participation in the initiation process. As a consequence, the oriC duplex may be transcriptionally activated to an open form, a necessary prerequisite for DNA polymerization.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference45 articles.

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5. Cashel M. and K. E. Rudd. 1987. The stringent response p. 1410-1438. In F. C. Neidhardt J. L. Ingraham K. B. Low B. Magasanik M. Schaechter and H. E. Umbarger (ed.) Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology Washington D.C.

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