Multiple defects in Escherichia coli mutants lacking HU protein-Reference-Cited by-同舟云学术

Multiple defects in Escherichia coli mutants lacking HU protein

Published:1989-07 Issue:7 Volume:171 Page:3704-3712
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Huisman O¹,Faelen M¹,Girard D¹,Jaffé A¹,Toussaint A¹,Rouvière-Yaniv J¹

Affiliation:

1. Département de Biotechnologie, Institut Pasteur, Paris, France.

Abstract

The HU protein isolated from Escherichia coli, composed of two partially homologous subunits, alpha and beta, shares some of the properties of eucaryotic histones and is a major constituent of the bacterial nucleoid. We report here the construction of double mutants totally lacking both subunits of HU protein. These mutants exhibited poor growth and a perturbation of cell division, resulting in the formation of anucleate cells. In the absence of HU, phage Mu was unable to grow, to lysogenize, or to carry out transposition.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.171.7.3704-3712.1989

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