Detection of 2,4,6-Trinitrotoluene-Utilizing Anaerobic Bacteria by 15 N and 13 C Incorporation-Reference-Cited by-同舟云学术

Detection of 2,4,6-Trinitrotoluene-Utilizing Anaerobic Bacteria by 15 N and 13 C Incorporation

Published:2010-03 Issue:5 Volume:76 Page:1695-1698
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Gallagher Erin M.¹,Young Lily Y.¹,McGuinness Lora M.²,Kerkhof Lee J.²

Affiliation:

1. Department of Environmental Sciences

2. Institute of Marine and Coastal Sciences, Rutgers, the State University of New Jersey, New Brunswick, New Jersey 08901

Abstract

ABSTRACT 2,4,6-Trinitrotoluene ( 15 N or 13 C labeled) was added to Norfolk Harbor sediments to test whether anaerobic bacteria use TNT for growth. Stable-isotope probing (SIP)-terminal restriction fragment length polymorphism (TRFLP) detected peaks in the [ 15 N]TNT cultures (60, 163, and 168 bp). The 60-bp peak was also present in the [ 13 C]TNT cultures and was related to Lysobacter taiwanensis .

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02274-09

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