Evaluation of the Roles of Four Candida albicans Genes in Virulence by Using Gene Disruption Strains That Express URA3 from the Native Locus-Reference-Cited by-同舟云学术

Evaluation of the Roles of Four Candida albicans Genes in Virulence by Using Gene Disruption Strains That Express URA3 from the Native Locus

Published:2003-10 Issue:10 Volume:71 Page:6101-6103
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Cheng Shaoji¹,Nguyen M. Hong¹²³,Zhang Zongde¹,Jia Hongyan¹,Handfield Martin⁴,Clancy Cornelius J.¹²³

Affiliation:

1. Departments of Medicine

2. Molecular Genetics and Microbiology, University of Florida College of Medicine

3. North Florida/South Georgia V.A. Medical Center, Gainesville, Florida 32608

4. Center for Molecular Microbiology and Department of Oral Biology, University of Florida College of Dentistry, Gainesville, Florida 32610

Abstract

ABSTRACT Reintroducing URA3 to its native locus in Candida albicans not5 , not3 , bur2 , and kel1 disruption mutants enabled us to directly compare strains with control strain CAI-12. We showed that URA3 position affected orotidine 5′-monophosphate decarboxylase activity, hyphal morphogenesis, adherence, and mortality in murine disseminated candidiasis. After URA3 was reintroduced to its native locus, only NOT5 could be conclusively ascribed a role in virulence.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.71.10.6101-6103.2003

Reference12 articles.

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