Rapid Detection of Sublethally Impaired Cells of Enterobacteriaceae in Dried Foods

Author:

Mossel D. A. A.1,Ratto M. Alina1

Affiliation:

1. Central Institute for Nutrition Research TNO, Zeist, The Netherlands, and San Marcos University, Lima, Peru

Abstract

Samples of dried foods, feeds, and drugs were examined for Enterobacteriaceae by using buffered glucose-Brilliant Green-bile broth as the final enrichment medium and deep tubes of violet-red-bile-glucose-agar for confirmation. The pre-enrichment treatments used for the resuscitation of sublethally impaired cells were overnight incubation in lactose broth versus incubation for 1 to 6 hr at room temperature in shallow layers of tryptone soya peptone broth. The latter restoration treatment appeared to be significantly more productive.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference12 articles.

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5. Lewicki P. P. and G. J. Silverman. 1969. Mechanisms concerned in the repair and recovery of sublethally impaired cells occurring in foods p. 106-134. In The microbiology of dried foods. Proc. 6th Int. Symp. Food Microbiol. Haarlem.

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