Isolation of Rhizobium phaseoli Tn5-induced mutants with altered expression of cytochrome terminal oxidases o and aa3

Author:

Soberón M1,Membrillo-Hernández J1,Aguilar G R1,Sánchez F1

Affiliation:

1. Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Cuernavaca, Morelos.

Abstract

Two Rhizobium phaseoli mutants affected in cytochrome expression were obtained by Tn5-mob mutagenesis of the wild-type strain (CE3). Mutant strain CFN031 expressed sevenfold less cytochrome o in culture, expressed cytochrome aa3 under microaerophilic culture conditions, in contrast to strain CE3, and was affected in its vegetative growth properties and proliferation inside plant host cells. Mutant CFN037 expressed cytochrome aa3 under microaerophilic culture conditions, while bacteroid development and nitrogen fixation occurred earlier than in strain CE3.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference24 articles.

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2. Electron transport systems of Rhizobium japonicum. II. Rhizobium haemoproteins, cytochromes and oxidases in free living (cultured) cells;Appleby C. A.;Biochim. Biophys. Acta,1969

3. Leghemoglobin and Rhizobium respiration. Annu;Appleby C. A.;Rev. Plant Physiol.,1984

4. Stimulation of tetrapyrrole formation in Rhizobiumjaponicum by restricted aeration;Avissar Y. J.;J. Bacteriol.,1978

5. The location of the enzymes of ammonia assimilation in root nodules of Phaseolus vulgaris. Plant Sci;Awoinaike K.;Lett.,1981

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