Properties of new Escherichia coli Hfr strains constructed by integration of pSC101-derived conjugative plasmids-Reference-Cited by-同舟云学术

Properties of new Escherichia coli Hfr strains constructed by integration of pSC101-derived conjugative plasmids

Published:1990-03 Issue:3 Volume:172 Page:1436-1440
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

François V¹,Conter A¹,Louarn J M¹

Affiliation:

1. Centre de Biochimie et de Génétique cellulaires du Centre National de la Recherche Scientifique, Toulouse, France.

Abstract

Conjugative temperature-sensitive plasmids were derived from pSC101. These plasmids are useful in genetic analysis for two reasons: (i) they render possible the construction of new Hfr lines by plasmid integration at predetermined chromosomal loci via Tn10 inverse transposition, and (ii) the Hfr characters are transducible via bacteriophage P1. We also showed that replication from pSC101 origin is deleterious for the plasmid-chromosome fusion.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.172.3.1436-1440.1990

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