Inhibition of Thiamine Pyrophosphate Utilization by Thiamine or Its Monophosphate in Escherichia coli

Author:

Nakayama Hideo1,Hayashi Ryoji1

Affiliation:

1. Department of Microbiology, Yamaguchi University School of Medicine, Ube, Yamaguchi-Ken, Japan

Abstract

The growth of a thiamine pyrophosphate auxotroph of Escherichi coli was inhibited by either thiamine or thiamine monophosphate, and the growth of a thiamine monophosphate auxotroph was inhibited by thiamine. The thiamine pyrophosphate-dependent oxidation of pyruvate was inhibited by thiamine with whole cells of the thiamine pyrophosphate auxotroph but not with cell extracts prepared from the same organism. In addition, the thiamine pyrophosphate uptake of the thiamine pyrophosphate auxotroph was inhibited by either thiamine or thiamine monophosphate. Although the thiamine pyrophosphate uptake of a revertant, selected for prototrophy from the thiamine monophosphate auxotroph, was inhibited by thiamine to an extent comparable to that observed with the thiamine monophosphate auxotroph, its growth was no longer inhibited by thiamine. A possible mechanism for the inhibition by thiamine and thiamine monophosphate in the utilization of thiamine pyrophosphate is discussed.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference20 articles.

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3. Mutants of Escherichia coli requiring methionine or vitamin B,2;Davis B. D.;J. Bacteriol.,1950

4. A resolution of the Escherichia coli pyruvate dehydrogenase complex;Gounaris A. D.;J. Biol. Chem.,1961

5. Thiamine-binding protein of Escherichia coli;Iwashima A.;J. Bacteriol.,1971

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