High Interlaboratory Reproducibility of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Species Identification of Nonfermenting Bacteria-Reference-Cited by-同舟云学术

High Interlaboratory Reproducibility of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Species Identification of Nonfermenting Bacteria

Published:2009-11 Issue:11 Volume:47 Page:3732-3734
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Mellmann A.¹,Bimet F.²,Bizet C.²,Borovskaya A. D.³,Drake R. R.⁴,Eigner U.⁵,Fahr A. M.⁵,He Y.⁶,Ilina E. N.³,Kostrzewa M.⁷,Maier T.⁷,Mancinelli L.⁸,Moussaoui W.⁹,Prévost G.⁹,Putignani L.⁸,Seachord C. L.⁴,Tang Y. W.⁶,Harmsen D.¹⁰

Affiliation:

1. Institute of Hygiene, University Hospital Muenster, D-48149 Muenster, Germany

2. Centre de Ressources Biologiques de l′Institut Pasteur, Department of Microbiology, Institut Pasteur, F-75724 Paris, France

3. Research Institute of Physical-Chemical Medicine, 119992 Moscow, Russia

4. Department of Microbiology and Molecular Cell Biology, Center for Biomedical Proteomics, Eastern Virginia Medical School, Norfolk, Virginia

5. Labor Limbach, 69126 Heidelberg, Germany

6. Departments of Medicine and Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232

7. Bruker Daltonik, GmbH, Bremen, Germany

8. Microbiology Unit, Bambino Gesù Children's Hospital, Health Care and Research Institute, 00165 Rome, Italy

9. Institut de Bactériologie UR Physiopathologie et Médicine Translationnelle, F-67000 Strasbourg, France

10. Department for Periodontology, University Hospital Muenster, D-48149 Muenster, Germany

Abstract

ABSTRACT Matrix-assisted laser desorption ionization-time of flight mass spectrometry has emerged as a rapid, cost-effective alternative for bacterial species identification. Identifying 60 blind-coded nonfermenting bacteria samples, this international study (using eight laboratories) achieved 98.75% interlaboratory reproducibility. Only 6 of the 480 samples were misidentified due to interchanges (4 samples) or contamination (1 sample) or not identified because of insufficient signal intensity (1 sample).

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00921-09

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