Evaluation of Three Real-Time PCR Assays for Detection of Mycoplasma pneumoniae in an Outbreak Investigation

Author:

Winchell Jonas M.1,Thurman Kathleen A.1,Mitchell Stephanie L.1,Thacker W. Lanier1,Fields Barry S.1

Affiliation:

1. Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia

Abstract

ABSTRACT We compared the performances of three recently optimized real-time PCR assays derived from distinct genomic regions of Mycoplasma pneumoniae during an outbreak. Comprehensive evaluation established that a newly described toxin gene represents a superior target for detecting M. pneumoniae DNA in clinical specimens, although use of multiple targets may increase testing confidence.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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