Affiliation:
1. Rockefeller University, New York, New York 10021.
Abstract
Understanding how diverse transcription patterns are achieved through common factor binding elements is a fundamental question that underlies much of developmental and cellular biology. One example is provided by the fibroblast growth factor 4 (FGF-4) gene, whose expression is restricted to specific embryonic tissues during development and to undifferentiated embryonal carcinoma cells in tissue culture. Analysis of the cis- and trans-acting elements required for the activity of the previously identified FGF-4 enhancer in F9 embryonal carcinoma cells showed that enhancer function depends on sequences that bind Sp1 and ubiquitous as well as F9-specific octamer-binding proteins. However, sequences immediately upstream of the octamer motif, which conform to a binding site for the high-mobility group (HMG) domain factor family, were also critical to enhancer function. We have identified a novel F9-specific factor, Fx, which specifically recognizes this motif. Fx formed complexes with either Oct-1 or Oct-3 in a template-dependent manner. The ability of different enhancer variants to form the Oct-Fx complexes correlated with enhancer activity, indicating that these complexes play an essential role in transcriptional activation of the FGF-4 gene. Thus, while FGF-4 enhancer function is octamer site dependent, its developmentally restricted activity is determined by the interaction of octamer-binding proteins with the tissue-specific factor Fx.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Reference79 articles.
1. The FGF family of growth factors and oncogenes;Basilico C.;Adv. Cancer Res.,1992
2. A common octamer binding protein is involved in the transcription of the U6 snRNA by polymerase III and U2 snRNA by RNA polymerase II;Carbon P.;Cell,1987
3. Oct-2, although not required for early B-cell development, is critical for later B-cell maturation and for postnatal survival;Corcoran L. M.;Genes Dev.,1993
4. Expression of the K-fgf proto-oncogene is controlled by 3' regulatory elements which are specific for embryonal carcinoma cells;Curatola A. M.;Mol. Cell. Biol.,1990
5. .Curatola A. M. and L. Dailey. Unpublished results.
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献