Affiliation:
1. Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 5531 Boelter Hall, Los Angeles, California 90095
Abstract
ABSTRACT
A synthetic pathway was engineered in
Escherichia coli
to produce isopropanol by expressing various combinations of genes from
Clostridium acetobutylicum
ATCC 824,
E. coli
K-12 MG1655,
Clostridium beijerinckii
NRRL B593, and
Thermoanaerobacter brockii
HTD4. The strain with the combination of
C. acetobutylicum thl
(acetyl-coenzyme A [CoA] acetyltransferase),
E. coli atoAD
(acetoacetyl-CoA transferase),
C. acetobutylicum adc
(acetoacetate decarboxylase), and
C. beijerinckii adh
(secondary alcohol dehydrogenase) achieved the highest titer. This strain produced 81.6 mM isopropanol in shake flasks with a yield of 43.5% (mol/mol) in the production phase. To our knowledge, this work is the first to produce isopropanol in
E. coli
, and the titer exceeded that from the native producers.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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