Serological Evidence of Human Klassevirus Infection-Reference-Cited by-同舟云学术

Serological Evidence of Human Klassevirus Infection

Published:2010-10 Issue:10 Volume:17 Page:1584-1588
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

Greninger Alexander L.¹²³⁴,Holtz Lori¹²³⁴,Kang Gagandeep¹²³⁴,Ganem Donald¹²³⁴,Wang David¹²³⁴,DeRisi Joseph L.¹²³⁴

Affiliation:

1. Howard Hughes Medical Institute, Departments of Medicine, Biochemistry and Biophysics, and Microbiology, University of California, San Francisco, California 94143

2. Department of Pediatrics, Washington University, St. Louis, Missouri 63110

3. Department of Gastrointestinal Sciences, Christian Medical College, Vellore, India

4. Departments of Molecular Microbiology and Pathology and Immunology, Washington University, St. Louis, Missouri 63110

Abstract

ABSTRACT Klassevirus is a proposed new genus of picornavirus that has been associated with pediatric diarrhea. In this study, we used recombinant klassevirus 3C protease as the capture antigen for an indirect serological enzyme-linked immunosorbent assay (ELISA). Four of six klassevirus reverse transcription (RT)-PCR-positive individuals demonstrated seroconversion against the 3C protease, suggesting that klassevirus infection and replication occur in humans. Additional screening of 353 samples from an age-banded serological cohort from two St. Louis hospitals indicated a seroprevalence of 6.8%.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CVI.00152-10

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