Anti-Protective Antigen IgG Enzyme-Linked Immunosorbent Assay for Diagnosis of Cutaneous Anthrax in India

Author:

Ghosh N.,Goel A. K.

Abstract

ABSTRACTAnthrax caused byBacillus anthracisis a public health problem in several developing countries whose main source of income is farming. Anthrax is a disease of herbivorous animals, and humans can be infected by handling infected animals or contaminated animal products. Specific diagnostic tests are unavailable in India for the detection and confirmation of cutaneous anthrax in humans. Here, we describe the development of an enzyme-linked immunosorbent assay (ELISA) for detection of serum antibodies againstBacillus anthracisprotective antigen in the Indian population. A total of 405 serum samples collected from different groups were tested by the developed ELISA. The assay provided a specificity of 99.41% (95% confidence interval [CI], 97.89 to 99.93) and a sensitivity of 100% (CI, 94.4 to 100) using a cutoff value of 0.29 ELISA unit (EU). The positive predictive value (PPV) and negative predictive value (NPV) of the assay were 97% and 100%, respectively. The efficiency and J index for the reliability of the assay were 99.5% and 0.994, respectively. The assay can be a very useful tool for surveillance as well as for diagnosis of cutaneous anthrax cases in India.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Reference30 articles.

1. Lethal factor toxemia and anti-protective antigen antibody activity in naturally acquired cutaneous anthrax;Boyer;J. Infect. Dis.,2011

2. Cutaneous anthrax in eastern Turkey;Caksen;Cutis,2001

3. Scrub typhus: radiological and clinical findings;Choi;Clin. Radiol.,2000

4. Three related cases of cutaneous anthrax in France: clinical and laboratory aspects;Cinquetti;Medicine (Baltimore),2009

5. Anthrax toxin;Collier;Annu. Rev. Cell Dev. Biol.,2003

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