Affiliation:
1. Quality Control Department, Statens Serum Institut, 5 Artillerivej, DK-2300 Copenhagen S, Denmark
Abstract
ABSTRACT
An interfering substance in various frozen serum samples was observed to inhibit the adhesion of Chinese hamster ovary (CHO) cells to microplate surfaces during a CHO pertussis neutralization test, resulting in wells that lacked cells or wells with dead cells after 2 days of incubation. The interfering activity in the serum could be eliminated by (i) transferring cells to other wells after their initial incubation, (ii) adding fetal calf serum (FCS) to the sample dilution buffer, (iii) precoating microplates with FCS, or (iv) preincubating the samples at 4°C for 5 days. Preincubating the samples at 4°C for 5 days reduced the interfering activity in only some of the samples. Adding serum to the sample dilution buffer or precoating the microplates with serum did not influence the antibody titers in the serum samples. The method described may be used for routine applications.
Publisher
American Society for Microbiology
Subject
Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy
Reference9 articles.
1. Gillenius, P., E. Jäätmaa, P. Askelöf, M. Granström, and M. Tiru. 1985. The standardization of an assay for pertussis toxin and antitoxin in microplate culture of Chinese hamster ovary cells. J. Biol. Stand.13:61-66.
2. Granström, M., G. Granström, P. Gillenius, and P. Askelöf. 1985. Neutralising antibodies to pertussis toxin in whooping cough. J. Infect. Dis.151:646-649.
3. Evaluation of serologic assays for diagnosis of whooping cough
4. Induction of a novel morphological response in Chinese hamster ovary cells by pertussis toxin
5. Melnick, J. L., H. A. Wenner, and C. A. Phillips. 1979. Enteroviruses, p. 471-534. In E. H. Lennette and N. J. Schmidt (ed.), Diagnostic procedures for viral, rickettsial and chlamydial infections. American Public Health Association, Inc., Washington, DC.