Affiliation:
1. Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907
Abstract
By using the method of stepwise homogenization of yeast spheroplast lysates employed previously with the leucine biosynthetic enzymes, it is shown that threonine deaminase, acetohydroxy acid synthase, Mg
2+
-dependent isomero-reductase, and dihydroxy acid dehydratase are particulate. Density gradient centrifugation and the behavior of marker enzymes suggest that all of the above enzymes of the isoleucine-valine biosynthetic pathway are associated with the mitochondria.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
121 articles.
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