Rapid Method for Detection and Enumeration of Fecal Coliforms in Fresh Chicken-Reference-Cited by-同舟云学术

Rapid Method for Detection and Enumeration of Fecal Coliforms in Fresh Chicken

Published:1974-06 Issue:6 Volume:27 Page:1127-1130
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Francis D. W.¹,Peeler J. T.¹,Twedt R. M.¹

Affiliation:

1. U.S. Department of Health, Education, and Welfare, Public Health Service, Food and Drug Administration, Division of Microbiology, Cincinnati, Ohio 45226

Abstract

A rapid method for enumerating fecal coliforms in foods was developed employing an agar pour-plate medium. After 7 h of incubation at 41.5 ± 0.05 C, this medium effectively allows the growth of fecal coliforms only. This rapid method was compared with the Association of Official Analytical Chemists multiple-tube dilution method for Escherichia coli , by using 21 samples of fresh, cut-up chicken and a surface rinsing procedure for sample preparation. Verification of picked colonies was carried out in EC broth using parallel incubation temperatures of 45.5 and 44.5 ± 0.05 C. Verifications for these temperatures averaged 79 and 98%, respectively. All positively verified isolates were E. coli types I and II, as were the negatives. Geometric means for the verified 7-h plate count were within 12% of the standard means for both EC broth incubation temperatures.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.27.6.1127-1130.1974

Reference17 articles.

1. Fluorescent antibody as a method for the detection of fecal pollution: Escherichia coli as indicator organism;Abshire R. L.;Can. J. Microbiol.,1973

2. American Public Health Association. 1958. Recommended methods for the microbiological examination of foods p. 124. American Public Health Association New York.

3. American Public Health Association. 1965. Standard methods for the examination of water and wastewater p. 599-600. American Public Health Association New York.

4. American Public Health Association. 1972. Standard methods for the examination of dairy products p. 56. American Public Health Association New York.

5. Rapid recovery of Escherichia coli from estuarine water;Andrews W. H.;Appl. Microbiol.,1972

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