Affiliation:
1. Department of Cell and Molecular Biology, University of Rhode Island, Kingston, Rhode Island, USA
2. Department of Microbiology and Plant Biology, University of Oklahoma, Norman, Oklahoma, USA
Abstract
ABSTRACT
Escherichia coli
MG1655, a K-12 strain, uses glycolytic nutrients exclusively to colonize the intestines of streptomycin-treated mice when it is the only
E. coli
strain present or when it is confronted with
E. coli
EDL933, an O157:H7 strain. In contrast,
E. coli
EDL933 uses glycolytic nutrients exclusively when it is the only
E. coli
strain in the intestine but switches in part to gluconeogenic nutrients when it colonizes mice precolonized with
E. coli
MG1655 (R. L. Miranda et al., Infect Immun 72:1666–1676, 2004,
http://dx.doi.org/10.1128/IAI.72.3.1666-1676.2004
). Recently, J. W. Njoroge et al. (mBio 3:e00280-12, 2012,
http://dx.doi.org/10.1128/mBio.00280-12
) reported that
E. coli
86-24, an O157:H7 strain, activates the expression of virulence genes under gluconeogenic conditions, suggesting that colonization of the intestine with a probiotic
E. coli
strain that outcompetes O157:H7 strains for gluconeogenic nutrients could render them nonpathogenic. Here we report that
E. coli
Nissle 1917, a probiotic strain, uses both glycolytic and gluconeogenic nutrients to colonize the mouse intestine between 1 and 5 days postfeeding, appears to stop using gluconeogenic nutrients thereafter in a large, long-term colonization niche, but continues to use them in a smaller niche to compete with invading
E. coli
EDL933. Evidence is also presented suggesting that invading
E. coli
EDL933 uses both glycolytic and gluconeogenic nutrients and needs the ability to perform gluconeogenesis in order to colonize mice precolonized with
E. coli
Nissle 1917. The data presented here therefore rule out the possibility that
E. coli
Nissle 1917 can starve the O157:H7
E. coli
strain EDL933 of gluconeogenic nutrients, even though
E. coli
Nissle 1917 uses such nutrients to compete with
E. coli
EDL933 in the mouse intestine.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
28 articles.
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