A Novel Staphylococcal Cassette Chromosome mec Type XI Primer for Detection of mecC -Harboring Methicillin-Resistant Staphylococcus aureus Directly from Screening Specimens-Reference-Cited by-同舟云学术

A Novel Staphylococcal Cassette Chromosome mec Type XI Primer for Detection of mecC -Harboring Methicillin-Resistant Staphylococcus aureus Directly from Screening Specimens

Published:2015-12 Issue:12 Volume:53 Page:3938-3941
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Petersdorf Sabine¹,Herma Miriam¹,Rosenblatt Meike¹,Layer Franziska²,Henrich Birgit¹

Affiliation:

1. Institute of Medical Microbiology and Hospital Hygiene, University Clinic of Heinrich-Heine University Düsseldorf, Düsseldorf, Germany

2. Robert Koch Institute, Department of Infectious Diseases, National Centre for Staphylococci and Enterococci, Wernigerode, Gemany

Abstract

ABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA) screening using real-time PCR has decreased in sensitivity due to the emergence of variant staphylococcal cassette chromosome mec element (SCC mec ) types. We have designed and validated a novel SCC mec XI primer, which enables for the first time the rapid detection of mecC -harboring MRSA directly from nasopharyngeal swabs without prior cultivation.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02328-15

Reference21 articles.

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