Affiliation:
1. Division of Microbiology and Veterinary Medicine, University of Wyoming, Laramie, Wyoming 82070, and Department of Dairy Science, University of Maryland, College Park, Maryland 20742
Abstract
Bacteroides amylophilus
has growth requirements for Na
+
, PO
4
3−
, K
+
, and small quantities of Mg
2+
. No requirement could be shown for Ca
2+
in media previously found growth-yield-limiting for
Bacteroides succinogenes
. Deletion of Co
2+
, Mn
2+
, Cl
−
, or SO
4
2−
did not affect growth. Quantitative studies indicate that Na
+
, K
+
, and PO
4
3−
have differing effects on the growth of
B. amylophilus
. A concentration of sodium and potassium ions affects both growth rate and growth yield, whereas a phosphate concentration markedly affects growth yield, but affects growth rate only slightly, if at all. The sodium requirement of
B. amylophilus
is absolute. It cannot be replaced by K
+
, Li
+
, Rb
+
, or Cs
+
. The latter three monovalent cations are toxic to
B. amylophilus
if supplied to the organism at Na
+
-replacing concentrations. K
+
is inactive at similar concentrations. The K
+
requirement of
B. amylophilus
may be satisfied by Rb
+
. The concentration of Na
+
required by
B. amylophilus
for abundant growth suggests that
B. amylophilus
should be considered a slightly halophilic organism. The results suggest that Na
+
may be a more frequent requirement among terrestial bacteria obtained from relatively low-salt environments than has been previously believed.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
50 articles.
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